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cryo stem eels spectrum imaging (Gatan Inc)

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Gatan Inc cryo stem eels spectrum imaging

<t>Cryo-ADF-STEM</t> micrograph of polymeric nanoparticles showing bright central regions in the core of some nanoparticles (green arrows) that might suggest a high density region within an inner core of the nanoparticles. (a)–(b) comparison between under-focus BF-TEM and ADF-STEM of the same region. Yellow curves indicate layer 1 and orange curves indicate layer 2. (c) A dark halo is visible around the particles in the ADF-STEM image (blue arrow and curves) suggesting the presence of layer 3 and it being lower density than the core and the surrounding vitreous ice. d) Application of a high pass filter to the ADF-STEM image removes thickness variation and emphasizes the low electron density of layer 3 and e) inverting the contrast of the high pass filtered ADF-STEM image reveals all three layers of the core-shell-corona nanoparticles. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Cryo Stem Eels Spectrum Imaging, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 98/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cryo+stem+eels+spectrum+imaging/STEMPack+Spectrum+Imaging/pmc10285183-58-0-7
Average 98 stars, based on 18 article reviews

cryo stem eels spectrum imaging - by Bioz Stars, 2026-11

98/100 stars

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1) Product Images from "Structure of polymeric nanoparticles encapsulating a drug – pamoic acid ion pair by scanning transmission electron microscopy"

Article Title: Structure of polymeric nanoparticles encapsulating a drug – pamoic acid ion pair by scanning transmission electron microscopy

Journal: Heliyon

doi: 10.1016/j.heliyon.2023.e16959

Cryo-ADF-STEM micrograph of polymeric nanoparticles showing bright central regions in the core of some nanoparticles (green arrows) that might suggest a high density region within an inner core of the nanoparticles. (a)–(b) comparison between under-focus BF-TEM and ADF-STEM of the same region. Yellow curves indicate layer 1 and orange curves indicate layer 2. (c) A dark halo is visible around the particles in the ADF-STEM image (blue arrow and curves) suggesting the presence of layer 3 and it being lower density than the core and the surrounding vitreous ice. d) Application of a high pass filter to the ADF-STEM image removes thickness variation and emphasizes the low electron density of layer 3 and e) inverting the contrast of the high pass filtered ADF-STEM image reveals all three layers of the core-shell-corona nanoparticles. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Figure Legend Snippet: Cryo-ADF-STEM micrograph of polymeric nanoparticles showing bright central regions in the core of some nanoparticles (green arrows) that might suggest a high density region within an inner core of the nanoparticles. (a)–(b) comparison between under-focus BF-TEM and ADF-STEM of the same region. Yellow curves indicate layer 1 and orange curves indicate layer 2. (c) A dark halo is visible around the particles in the ADF-STEM image (blue arrow and curves) suggesting the presence of layer 3 and it being lower density than the core and the surrounding vitreous ice. d) Application of a high pass filter to the ADF-STEM image removes thickness variation and emphasizes the low electron density of layer 3 and e) inverting the contrast of the high pass filtered ADF-STEM image reveals all three layers of the core-shell-corona nanoparticles. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Techniques Used:

(a) Under-focus BF-TEM image and PCA treated carbon STEM-EELS mapping of the area marked in red. (b) Under-focus BF-TEM, ADF-STEM and C- K EELS mapping of a cropped fragment marked in yellow in (a) and showing carbon presence between the particles, confirming the C-content to layer 3. The corresponding diameter of layer 1 + 2 is identical between cropped under-focus BF-TEM, ADF-STEM and C- K map images (yellow arrows) and the thickness of layer 3 is consistent with earlier measurements of the spacing between nanoparticles by under-focus BF-TEM (blue arrow). Red arrows indicate severe electron beam damage in layer 2 visible in a post-EELS acquisition, ADF-STEM image. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Figure Legend Snippet: (a) Under-focus BF-TEM image and PCA treated carbon STEM-EELS mapping of the area marked in red. (b) Under-focus BF-TEM, ADF-STEM and C- K EELS mapping of a cropped fragment marked in yellow in (a) and showing carbon presence between the particles, confirming the C-content to layer 3. The corresponding diameter of layer 1 + 2 is identical between cropped under-focus BF-TEM, ADF-STEM and C- K map images (yellow arrows) and the thickness of layer 3 is consistent with earlier measurements of the spacing between nanoparticles by under-focus BF-TEM (blue arrow). Red arrows indicate severe electron beam damage in layer 2 visible in a post-EELS acquisition, ADF-STEM image. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Techniques Used:

(a) Cryo-ADF-STEM image of particles and areas where spectra were extracted from – layer 1 (yellow box), layer 2 (orange boxes), layer 3 (blue boxes) and amorphous ice (purple box). (b) Background stripped, as-acquired (faint dots) and smoothed (bold lines) STEM-EELS from layer 1, layer 2 layer 3 and amorphous ice areas in (a). Layer 1 is C rich and also has a clear N peak identifiable. Layer 2 and 3 have progressively less C and relatively more O per layer and less than that of the amorphous ice. (c) Background stripped, as-acquired (faint lines) and smoothed (bold lines) STEM-EELS at the C- K edge reveal that layer 2 and layer 3 have slightly different edge structure to that of layer 1 suggesting a different polymer composition. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Figure Legend Snippet: (a) Cryo-ADF-STEM image of particles and areas where spectra were extracted from – layer 1 (yellow box), layer 2 (orange boxes), layer 3 (blue boxes) and amorphous ice (purple box). (b) Background stripped, as-acquired (faint dots) and smoothed (bold lines) STEM-EELS from layer 1, layer 2 layer 3 and amorphous ice areas in (a). Layer 1 is C rich and also has a clear N peak identifiable. Layer 2 and 3 have progressively less C and relatively more O per layer and less than that of the amorphous ice. (c) Background stripped, as-acquired (faint lines) and smoothed (bold lines) STEM-EELS at the C- K edge reveal that layer 2 and layer 3 have slightly different edge structure to that of layer 1 suggesting a different polymer composition. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Techniques Used:

Figure Legend Snippet: (a) Cryo-ADF STEM and cryo-STEM EELS carbon and nitrogen mapping. (b) Cryo-STEM-EEL spectrum acquired over the whole area shows the presence of C, N and O. (c) A linear intensity profile taken from the C–N overlay map and ADF-STEM image indicates that C occurs also between the nanoparticles and N is located approximately in the centre of individual nanoparticles (yellow box in (a)). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Techniques Used:

(a) Under-focus BF-TEM of non-standard nanoparticles named as: debris (non-spherical particles, marked as orange arrows) and blank (no visible layer 1, marked as red arrows). Both non-standard particles are highly beam sensitive. (b) Cropped cryo-STEM-EELS mapping of area marked in yellow in (a) suggests that debris and blank particles consist of the same material as layer 2 in the standard particle (i.e., have negligible N content and are highly beam sensitive). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Figure Legend Snippet: (a) Under-focus BF-TEM of non-standard nanoparticles named as: debris (non-spherical particles, marked as orange arrows) and blank (no visible layer 1, marked as red arrows). Both non-standard particles are highly beam sensitive. (b) Cropped cryo-STEM-EELS mapping of area marked in yellow in (a) suggests that debris and blank particles consist of the same material as layer 2 in the standard particle (i.e., have negligible N content and are highly beam sensitive). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Techniques Used:

(a) Final multi-modal data-driven model of the polymeric nanoparticles based on (i) ADF-STEM (*high-pass filtered), (ii) phase contrast-TEM and (iii) STEM-EELS analysis. (b) The likely content of each layer is -pamoic acid-API and some PLA material in layer 1 with a core enriched in pamoic acid-API material. Only PEG and PLA in layer 2 and diffuse PEG packing in layer 3.

Figure Legend Snippet: (a) Final multi-modal data-driven model of the polymeric nanoparticles based on (i) ADF-STEM (*high-pass filtered), (ii) phase contrast-TEM and (iii) STEM-EELS analysis. (b) The likely content of each layer is -pamoic acid-API and some PLA material in layer 1 with a core enriched in pamoic acid-API material. Only PEG and PLA in layer 2 and diffuse PEG packing in layer 3.

Techniques Used:

Imaging:

Article Title: Structure of polymeric nanoparticles encapsulating a drug – pamoic acid ion pair by scanning transmission electron microscopy
Article Snippet: .. Cryo-STEM-EELS spectrum imaging was undertaken using the Gatan Quantum 965 ER imaging filter in the image-coupled mode. ..

Image Search Results

Journal: Heliyon

Article Title: Structure of polymeric nanoparticles encapsulating a drug – pamoic acid ion pair by scanning transmission electron microscopy

doi: 10.1016/j.heliyon.2023.e16959

Figure Lengend Snippet: Cryo-ADF-STEM micrograph of polymeric nanoparticles showing bright central regions in the core of some nanoparticles (green arrows) that might suggest a high density region within an inner core of the nanoparticles. (a)–(b) comparison between under-focus BF-TEM and ADF-STEM of the same region. Yellow curves indicate layer 1 and orange curves indicate layer 2. (c) A dark halo is visible around the particles in the ADF-STEM image (blue arrow and curves) suggesting the presence of layer 3 and it being lower density than the core and the surrounding vitreous ice. d) Application of a high pass filter to the ADF-STEM image removes thickness variation and emphasizes the low electron density of layer 3 and e) inverting the contrast of the high pass filtered ADF-STEM image reveals all three layers of the core-shell-corona nanoparticles. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Cryo-STEM-EELS spectrum imaging was undertaken using the Gatan Quantum 965 ER imaging filter in the image-coupled mode.

Techniques:

Journal: Heliyon

Article Title: Structure of polymeric nanoparticles encapsulating a drug – pamoic acid ion pair by scanning transmission electron microscopy

doi: 10.1016/j.heliyon.2023.e16959

Figure Lengend Snippet: (a) Under-focus BF-TEM image and PCA treated carbon STEM-EELS mapping of the area marked in red. (b) Under-focus BF-TEM, ADF-STEM and C- K EELS mapping of a cropped fragment marked in yellow in (a) and showing carbon presence between the particles, confirming the C-content to layer 3. The corresponding diameter of layer 1 + 2 is identical between cropped under-focus BF-TEM, ADF-STEM and C- K map images (yellow arrows) and the thickness of layer 3 is consistent with earlier measurements of the spacing between nanoparticles by under-focus BF-TEM (blue arrow). Red arrows indicate severe electron beam damage in layer 2 visible in a post-EELS acquisition, ADF-STEM image. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Cryo-STEM-EELS spectrum imaging was undertaken using the Gatan Quantum 965 ER imaging filter in the image-coupled mode.

Techniques:

Journal: Heliyon

Article Title: Structure of polymeric nanoparticles encapsulating a drug – pamoic acid ion pair by scanning transmission electron microscopy

doi: 10.1016/j.heliyon.2023.e16959

Figure Lengend Snippet: (a) Cryo-ADF-STEM image of particles and areas where spectra were extracted from – layer 1 (yellow box), layer 2 (orange boxes), layer 3 (blue boxes) and amorphous ice (purple box). (b) Background stripped, as-acquired (faint dots) and smoothed (bold lines) STEM-EELS from layer 1, layer 2 layer 3 and amorphous ice areas in (a). Layer 1 is C rich and also has a clear N peak identifiable. Layer 2 and 3 have progressively less C and relatively more O per layer and less than that of the amorphous ice. (c) Background stripped, as-acquired (faint lines) and smoothed (bold lines) STEM-EELS at the C- K edge reveal that layer 2 and layer 3 have slightly different edge structure to that of layer 1 suggesting a different polymer composition. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Cryo-STEM-EELS spectrum imaging was undertaken using the Gatan Quantum 965 ER imaging filter in the image-coupled mode.

Techniques:

Journal: Heliyon

Article Title: Structure of polymeric nanoparticles encapsulating a drug – pamoic acid ion pair by scanning transmission electron microscopy

doi: 10.1016/j.heliyon.2023.e16959

Figure Lengend Snippet: (a) Cryo-ADF STEM and cryo-STEM EELS carbon and nitrogen mapping. (b) Cryo-STEM-EEL spectrum acquired over the whole area shows the presence of C, N and O. (c) A linear intensity profile taken from the C–N overlay map and ADF-STEM image indicates that C occurs also between the nanoparticles and N is located approximately in the centre of individual nanoparticles (yellow box in (a)). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Cryo-STEM-EELS spectrum imaging was undertaken using the Gatan Quantum 965 ER imaging filter in the image-coupled mode.

Techniques:

Journal: Heliyon

Article Title: Structure of polymeric nanoparticles encapsulating a drug – pamoic acid ion pair by scanning transmission electron microscopy

doi: 10.1016/j.heliyon.2023.e16959

Figure Lengend Snippet: (a) Under-focus BF-TEM of non-standard nanoparticles named as: debris (non-spherical particles, marked as orange arrows) and blank (no visible layer 1, marked as red arrows). Both non-standard particles are highly beam sensitive. (b) Cropped cryo-STEM-EELS mapping of area marked in yellow in (a) suggests that debris and blank particles consist of the same material as layer 2 in the standard particle (i.e., have negligible N content and are highly beam sensitive). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Cryo-STEM-EELS spectrum imaging was undertaken using the Gatan Quantum 965 ER imaging filter in the image-coupled mode.

Techniques:

Journal: Heliyon

Article Title: Structure of polymeric nanoparticles encapsulating a drug – pamoic acid ion pair by scanning transmission electron microscopy

doi: 10.1016/j.heliyon.2023.e16959

Figure Lengend Snippet: (a) Final multi-modal data-driven model of the polymeric nanoparticles based on (i) ADF-STEM (*high-pass filtered), (ii) phase contrast-TEM and (iii) STEM-EELS analysis. (b) The likely content of each layer is -pamoic acid-API and some PLA material in layer 1 with a core enriched in pamoic acid-API material. Only PEG and PLA in layer 2 and diffuse PEG packing in layer 3.

Article Snippet: Cryo-STEM-EELS spectrum imaging was undertaken using the Gatan Quantum 965 ER imaging filter in the image-coupled mode.

Techniques:

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cryo stem eels spectrum imaging (Gatan Inc)

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1) Product Images from "Structure of polymeric nanoparticles encapsulating a drug – pamoic acid ion pair by scanning transmission electron microscopy"

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